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Insulin-Responsive Compartments Containing GLUT4 in 3T3-L1 and CHO Cells: Regulation by Amino Acid Concentrations

In fat and muscle, insulin stimulates glucose uptake by rapidly mobilizing the GLUT4 glucose transporter from a specialized intracellular compartment to the plasma membrane. We describe a method to quantify the relative proportion of GLUT4 at the plasma membrane, using flow cytometry to measure a ra...

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Detalhes bibliográficos
Main Authors: Bogan, Jonathan S., McKee, Adrienne E., Lodish, Harvey F.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87167/
https://ncbi.nlm.nih.gov/pubmed/11416153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.21.14.4785-4806.2001
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