Novel Fluorescence-Based Screen To Identify Small Synthetic Internal Ribosome Entry Site Elements

We report here a novel fluorescent protein-based screen to identify small, synthetic internal ribosome entry site (IRES) elements in vivo. A library of bicistronic plasmids encoding the enhanced blue and green fluorescent proteins (EBFP and EGFP) separated by randomized 50-nucleotide-long sequences...

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Bibliographic Details
Main Authors: Venkatesan, Arun, Dasgupta, Asim
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Gene Expression
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC86912/
https://ncbi.nlm.nih.gov/pubmed/11283261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.21.8.2826-2837.2001
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