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Novel Fluorescence-Based Screen To Identify Small Synthetic Internal Ribosome Entry Site Elements
We report here a novel fluorescent protein-based screen to identify small, synthetic internal ribosome entry site (IRES) elements in vivo. A library of bicistronic plasmids encoding the enhanced blue and green fluorescent proteins (EBFP and EGFP) separated by randomized 50-nucleotide-long sequences...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2001
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC86912/ https://ncbi.nlm.nih.gov/pubmed/11283261 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.21.8.2826-2837.2001 |
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