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Direct PCR of Cryptococcus neoformans MATα and MATa Pheromones To Determine Mating Type, Ploidy, and Variety: a Tool for Epidemiological and Molecular Pathogenesis Studies

Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally,...

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Udgivet i:J Clin Microbiol
Principais autores: Chaturvedi, Sudha, Rodeghier, Birgit, Fan, Jinjiang, McClelland, Carol M., Wickes, Brian L., Chaturvedi, Vishnu
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Mycology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86654/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790143/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.2007-2009.2000
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