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Direct PCR of Cryptococcus neoformans MATα and MATa Pheromones To Determine Mating Type, Ploidy, and Variety: a Tool for Epidemiological and Molecular Pathogenesis Studies

Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally,...

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Publicado en:J Clin Microbiol
Principais autores: Chaturvedi, Sudha, Rodeghier, Birgit, Fan, Jinjiang, McClelland, Carol M., Wickes, Brian L., Chaturvedi, Vishnu
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2000
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86654/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790143/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.2007-2009.2000
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