Direct PCR of Cryptococcus neoformans MATα and MATa Pheromones To Determine Mating Type, Ploidy, and Variety: a Tool for Epidemiological and Molecular Pathogenesis Studies

Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally,...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Chaturvedi, Sudha, Rodeghier, Birgit, Fan, Jinjiang, McClelland, Carol M., Wickes, Brian L., Chaturvedi, Vishnu
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Mycology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86654/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790143/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.2007-2009.2000
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