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Multiplex PCRs for Identification of Escherichia coli Virulence Genes

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Pass, M. A., Odedra, R., Batt, R. M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86652/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790141/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.2001-2004.2000
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