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Multiplex PCRs for Identification of Escherichia coli Virulence Genes

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These...

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Bibliographic Details
Main Authors: Pass, M. A., Odedra, R., Batt, R. M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC86652/
https://ncbi.nlm.nih.gov/pubmed/10790141
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