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Multiplex PCRs for Identification of Escherichia coli Virulence Genes
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These...
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Main Authors: | , , |
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格式: | Artigo |
語言: | Inglês |
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American Society for Microbiology
2000
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在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC86652/ https://ncbi.nlm.nih.gov/pubmed/10790141 |
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