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Characterization of a Vacuolar Pyrophosphatase in Trypanosoma brucei and Its Localization to Acidocalcisomes

Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized procyclic trypomastigotes of Trypanosoma brucei, as measured by acridine orange uptake. The proton gradient generated by pyrophosphate was collapsed by addition of nigericin or NH(4)Cl. Pyrophosphate-...

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Detalles Bibliográficos
Main Authors: Rodrigues, Claudia O., Scott, David A., Docampo, Roberto
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC84816/
https://ncbi.nlm.nih.gov/pubmed/10523660
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