Rapid Identification of up to Three Candida Species in a Single Reaction Tube by a 5′ Exonuclease Assay Using Fluorescent DNA Probes
We used fungus-specific PCR primers and species-specific DNA probes to detect up to three Candida species in a single reaction tube by exploiting the 5′ to 3′ exonuclease activity of Taq DNA polymerase. Probes to the internal transcribed spacer region of the rRNA gene were labeled at the 5′ end with...
Guardado en:
| Publicado en: | J Clin Microbiol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1999
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84197/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9854084/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.1.165-170.1999 |
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