Rapid Identification of up to Three Candida Species in a Single Reaction Tube by a 5′ Exonuclease Assay Using Fluorescent DNA Probes

We used fungus-specific PCR primers and species-specific DNA probes to detect up to three Candida species in a single reaction tube by exploiting the 5′ to 3′ exonuclease activity of Taq DNA polymerase. Probes to the internal transcribed spacer region of the rRNA gene were labeled at the 5′ end with...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Shin, Jong Hee, Nolte, Frederick S., Holloway, Brian P., Morrison, Christine J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Mycology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84197/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9854084/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.1.165-170.1999
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