Rapid Identification of up to Three Candida Species in a Single Reaction Tube by a 5′ Exonuclease Assay Using Fluorescent DNA Probes

We used fungus-specific PCR primers and species-specific DNA probes to detect up to three Candida species in a single reaction tube by exploiting the 5′ to 3′ exonuclease activity of Taq DNA polymerase. Probes to the internal transcribed spacer region of the rRNA gene were labeled at the 5′ end with...

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Main Authors: Shin, Jong Hee, Nolte, Frederick S., Holloway, Brian P., Morrison, Christine J.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
Teme:
Mycology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC84197/
https://ncbi.nlm.nih.gov/pubmed/9854084
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