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Transcriptional and Posttranscriptional Silencing of Rodent α1(I) Collagen by a Homologous Transcriptionally Self-Silenced Transgene

Transient transfection of rodent fibroblasts with plasmids carrying a full-size pro-α1(I) collagen gene (pWTC1) results in rapid reduction of the endogenous transcripts by >90%, while the transgene mRNA is undetectable. Using deletion constructs, two adjacent 5′ noncoding regions of the gene are...

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Autors principals: Bahramian, Mohammad B., Zarbl, Helmut
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC83885/
https://ncbi.nlm.nih.gov/pubmed/9858551
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