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Protein-Observed Fluorine NMR Is a Complementary Ligand Discovery Method to (1)H CPMG Ligand-Observed NMR

To evaluate its potential as a ligand discovery tool, we compare a newly developed 1D protein-observed fluorine NMR (PrOF NMR) screening method with the well-characterized ligand-observed (1)H CPMG NMR screen. We selected the first bromodomain of Brd4 as a model system to benchmark PrOF NMR because...

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Bibliografske podrobnosti
izdano v:ACS Chem Biol
Main Authors: Urick, Andrew K., Calle, Luis Pablo, Espinosa, Juan F., Hu, Haitao, Pomerantz, William C. K.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC8325173/
https://ncbi.nlm.nih.gov/pubmed/27627661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.6b00730
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