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Base editing-coupled survival screening enabled high-sensitive analysis of PAM compatibility and finding of the new possible off-target
Base editing (BE) is a promising genome engineering tool for modifying DNA or RNA and has been widely used in various microorganisms as well as eukaryotic cells. Despite the proximal protospacer adjacent motif (PAM) is critical to the targeting range and off-target effect of BE, there is still lack...
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| Publicado no: | iScience |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8324807/ https://ncbi.nlm.nih.gov/pubmed/34337358 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2021.102769 |
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