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Improvement in extracellular secretion of recombinant l-asparaginase II by Escherichia coli BL21 (DE3) using glycine and n-dodecane

l-asparaginase II (ASNase) is the biopharmaceutical of choice for the treatment of acute lymphoblastic leukaemia. In this study, E. coli BL21 (DE3) transformed with the pET15b + asnB vector which expresses recombinant ASNase was used as a source to obtain this enzyme. The ideal conditions to produce...

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Détails bibliographiques
Publié dans:Braz J Microbiol
Auteurs principaux: Flores-Santos, Juan Carlos, Moguel, Ignacio S., Monteiro, Gisele, Pessoa, Adalberto, Vitolo, Michele
Format: Artigo
Langue:Inglês
Publié: Springer International Publishing 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8324600/
https://ncbi.nlm.nih.gov/pubmed/34100260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s42770-021-00534-y
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