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Efficient low-cost chromatin profiling with CUT&Tag
We recently introduced CUT&Tag, an epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in permeabilized nuclei, and then used to tether the cut-and-paste transposase Tn5. Activation of the transposase simultaneously cleaves DNA and adds adapters (“tagmentati...
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| Published in: | Nat Protoc |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8318778/ https://ncbi.nlm.nih.gov/pubmed/32913232 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0373-x |
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