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Efficient low-cost chromatin profiling with CUT&Tag

We recently introduced CUT&Tag, an epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in permeabilized nuclei, and then used to tether the cut-and-paste transposase Tn5. Activation of the transposase simultaneously cleaves DNA and adds adapters (“tagmentati...

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Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Kaya-Okur, Hatice S., Janssens, Derek H., Henikoff, Jorja G., Ahmad, Kami, Henikoff, Steven
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC8318778/
https://ncbi.nlm.nih.gov/pubmed/32913232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0373-x
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