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Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops

The PcrA/UvrD helicase binds directly to RNA polymerase (RNAP) but the structural basis for this interaction and its functional significance have remained unclear. In this work, we used biochemical assays and hydrogen-deuterium exchange coupled to mass spectrometry to study the PcrA-RNAP complex. We...

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Détails bibliographiques
Publié dans:eLife
Auteurs principaux: Urrutia-Irazabal, Inigo, Ault, James R, Sobott, Frank, Savery, Nigel J, Dillingham, Mark S
Format: Artigo
Langue:Inglês
Publié: eLife Sciences Publications, Ltd 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8318588/
https://ncbi.nlm.nih.gov/pubmed/34279225
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.68829
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