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Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops

The PcrA/UvrD helicase binds directly to RNA polymerase (RNAP) but the structural basis for this interaction and its functional significance have remained unclear. In this work, we used biochemical assays and hydrogen-deuterium exchange coupled to mass spectrometry to study the PcrA-RNAP complex. We...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Urrutia-Irazabal, Inigo, Ault, James R, Sobott, Frank, Savery, Nigel J, Dillingham, Mark S
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8318588/
https://ncbi.nlm.nih.gov/pubmed/34279225
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.68829
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