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Photocrosslinking O-GlcNAcylated proteins to neighboring biomolecules
This protocol enables identification of the interaction partners of O-GlcNAcylated proteins. The method involves the introduction of the diazirine photocrosslinker onto the O-GlcNAc modification within living cells. The photocrosslinker is activated by UV light to yield covalent crosslinking between...
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| 出版年: | Curr Protoc |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2021
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8299865/ https://ncbi.nlm.nih.gov/pubmed/34288588 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.201 |
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