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Photocrosslinking O-GlcNAcylated proteins to neighboring biomolecules

This protocol enables identification of the interaction partners of O-GlcNAcylated proteins. The method involves the introduction of the diazirine photocrosslinker onto the O-GlcNAc modification within living cells. The photocrosslinker is activated by UV light to yield covalent crosslinking between...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Curr Protoc
Egile Nagusiak: Capota, Emanuela, Wu, Han, Kohler, Jennifer J.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2021
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC8299865/
https://ncbi.nlm.nih.gov/pubmed/34288588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.201
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