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Photocrosslinking O-GlcNAcylated proteins to neighboring biomolecules
This protocol enables identification of the interaction partners of O-GlcNAcylated proteins. The method involves the introduction of the diazirine photocrosslinker onto the O-GlcNAc modification within living cells. The photocrosslinker is activated by UV light to yield covalent crosslinking between...
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| Gepubliceerd in: | Curr Protoc |
|---|---|
| Hoofdauteurs: | , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2021
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8299865/ https://ncbi.nlm.nih.gov/pubmed/34288588 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.201 |
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