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Optogenetic control of calcium influx in mammalian cells

Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) ch...

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Bibliographic Details
Published in:Methods Enzymol
Main Authors: Lee, Yi-Tsang, Chen, Rui, Zhou, Yubin, He, Lian
Format: Artigo
Language:Inglês
Published: 2021
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8290970/
https://ncbi.nlm.nih.gov/pubmed/34120716
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2021.02.010
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