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Characterization of Protein Domain Function via in vitro DNA Shuffling

We recently investigated the molecular events that drive evolution of the CTX-M-type β-lactamases by DNA shuffling of fragments of the bla(CTX-M-14) and bla(CTX-M-15 )genes. Analysis of a total of 51 hybrid enzymes showed that enzymatic activity could be maintained in most cases, yet the enzymatical...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Po, Kathy Hiu Laam, Chan, Edward Wai Chi, Chen, Sheng
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8275271/
https://ncbi.nlm.nih.gov/pubmed/34285987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2873
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