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Characterization of Protein Domain Function via in vitro DNA Shuffling
We recently investigated the molecular events that drive evolution of the CTX-M-type β-lactamases by DNA shuffling of fragments of the bla(CTX-M-14) and bla(CTX-M-15 )genes. Analysis of a total of 51 hybrid enzymes showed that enzymatic activity could be maintained in most cases, yet the enzymatical...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8275271/ https://ncbi.nlm.nih.gov/pubmed/34285987 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2873 |
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