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Engineering of the genome editing protein Cas9 to slide along DNA

The genome editing protein Cas9 faces engineering challenges in improving off–target DNA cleavage and low editing efficiency. In this study, we aimed to engineer Cas9 to be able to slide along DNA, which might facilitate genome editing and reduce off-target cleavage. We used two approaches to achiev...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Banerjee, Trishit, Takahashi, Hiroto, Subekti, Dwiky Rendra Graha, Kamagata, Kiyoto
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8266852/
https://ncbi.nlm.nih.gov/pubmed/34239016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-93685-9
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