Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers

In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic microbiology. We also investigate the importance of sequencing depth. We first...

Cur síos iomlán

Na minha lista:
Sonraí Bibleagrafaíochta
Foilsithe in:mBio
Main Authors: Dyrhovden, Ruben, Rippin, Martin, Øvrebø, Kjell Kåre, Nygaard, Randi M., Ulvestad, Elling, Kommedal, Øyvind
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2021
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8262989/
https://ncbi.nlm.nih.gov/pubmed/34101489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.00598-21
Clibeanna: Cuir Clib Leis
Gan Chlibeanna, Bí ar an gcéad duine leis an taifead seo a chlibeáil!

Míreanna Comhchosúla