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Simultaneous Sequence Analysis of the 16S rRNA and rpoB Genes by Use of RipSeq Software To Identify Mycobacterium Species

The 16S rRNA gene is commonly used to identify Mycobacterium spp., but alternative DNA targets can provide better resolution to the species level. We evaluated a novel system that enables simultaneous amplification, sequencing, and analysis of two different DNA targets in a single tube to identify c...

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Detalhes bibliográficos
Main Authors: Simmon, Keith E., Kommedal, Øyvind, Saebo, Øystein, Karlsen, Bjarte, Petti, Cathy A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937733/
https://ncbi.nlm.nih.gov/pubmed/20610683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00362-10
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