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Simultaneous Sequence Analysis of the 16S rRNA and rpoB Genes by Use of RipSeq Software To Identify Mycobacterium Species

The 16S rRNA gene is commonly used to identify Mycobacterium spp., but alternative DNA targets can provide better resolution to the species level. We evaluated a novel system that enables simultaneous amplification, sequencing, and analysis of two different DNA targets in a single tube to identify c...

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Autors principals: Simmon, Keith E., Kommedal, Øyvind, Saebo, Øystein, Karlsen, Bjarte, Petti, Cathy A.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937733/
https://ncbi.nlm.nih.gov/pubmed/20610683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00362-10
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