Deep profiling of microgram-scale proteome by Tandem Mass Tag Mass Spectrometry

Tandem mass tag (TMT)-based mass spectrometry (MS) enables deep proteomic profiling of more than 10,000 proteins in complex biological samples, but requires up to 100 micrograms protein in starting materials during a standard analysis. Here we present a streamlined protocol to quantify more than 9,0...

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Vydáno v:J Proteome Res
Hlavní autoři: Liu, Danting, Yang, Shu, Kavdia, Kanisha, Sifford, Jeffrey M., Wu, Zhiping, Xie, Boer, Wang, Zhen, Pagala, Vishwajeeth R., Wang, Hong, Yu, Kaiwen, Dey, Kaushik Kumar, High, Anthony A., Serrano, Geidy E., Beach, Thomas G., Peng, Junmin
Médium: Artigo
Jazyk:Inglês
Vydáno: 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8262114/
https://ncbi.nlm.nih.gov/pubmed/33175545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.0c00426
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