Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Many exceptional advances have been made in mass spectrometry (MS)-based proteomics, with particular technical progress in liquid chromatography (LC) coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep-proteomics profiling protocol th...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: High, Anthony A., Tan, Haiyan, Pagala, Vishwajeeth R., Niu, Mingming, Cho, Ji-Hoon, Wang, Xusheng, Bai, Bing, Peng, Junmin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755397/
https://ncbi.nlm.nih.gov/pubmed/29286450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56474
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