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In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Shen, Shensi, Girault, Isabelle, Malka-Mahieu, Hélène, Robert, Caroline, Vagner, Stéphan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8243118/
https://ncbi.nlm.nih.gov/pubmed/34223196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100621
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