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Using RNA-binding proteins for immunoprecipitation of mRNAs from Xenopus laevis embryos
This protocol is developed for identifying mRNAs that form complexes with mRNA-binding proteins (mRBPs) in Xenopus laevis embryos at different developmental stages. Here, we describe the use of the Ybx1 mRBP for immunoprecipitation-based mRNA isolation. This protocol features the translation of the...
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| Vydáno v: | STAR Protoc |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Elsevier
2021
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8233257/ https://ncbi.nlm.nih.gov/pubmed/34195668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100552 |
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