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Protocol for separation of the nuclear and the cytoplasmic fractions of Xenopus laevis embryonic cells for studying protein shuttling

This protocol for the separation of nuclear and cytoplasmic fractions of cells of Xenopus laevis embryos was developed to study changes in the intracellular localization of the Zyxin and Ybx1 proteins, which are capable of changing localization in response to certain stimuli. Western blot analysis a...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Martynova, Natalia Y., Parshina, Elena A., Zaraisky, Andrey G.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8091922/
https://ncbi.nlm.nih.gov/pubmed/33997802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100449
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