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Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli

With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology since PCR. The key advantage of this method is its simplicity and universal applicability for specie...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: König, Enrico, Zerbini, Francesca, Zanella, Ilaria, Fraccascia, Davide, Grandi, Guido
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203979/
https://ncbi.nlm.nih.gov/pubmed/34179238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2688
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