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Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli

With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology since PCR. The key advantage of this method is its simplicity and universal applicability for specie...

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Dades bibliogràfiques
Publicat a:Bio Protoc
Autors principals: König, Enrico, Zerbini, Francesca, Zanella, Ilaria, Fraccascia, Davide, Grandi, Guido
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203979/
https://ncbi.nlm.nih.gov/pubmed/34179238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2688
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