Načítá se...

Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay

This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are immunoprecipitated in HA-affinity resin. Cell extracts are treated with nucl...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Bio Protoc
Hlavní autoři: Lechuga, Ana, Berjón-Otero, Mónica, Salas, Margarita, Redrejo-Rodríguez, Modesto
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203933/
https://ncbi.nlm.nih.gov/pubmed/34179232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2678
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!