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Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining

Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Bio Protoc
Prif Awduron: Juengert, Janina R., Bresan, Stephanie, Jendrossek, Dieter
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Bio-Protocol 2018
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203877/
https://ncbi.nlm.nih.gov/pubmed/34179275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2748
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