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Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining

Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to...

詳細記述

保存先:
書誌詳細
出版年:Bio Protoc
主要な著者: Juengert, Janina R., Bresan, Stephanie, Jendrossek, Dieter
フォーマット: Artigo
言語:Inglês
出版事項: Bio-Protocol 2018
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203877/
https://ncbi.nlm.nih.gov/pubmed/34179275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2748
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