Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining

Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Juengert, Janina R., Bresan, Stephanie, Jendrossek, Dieter
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203877/
https://ncbi.nlm.nih.gov/pubmed/34179275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2748
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