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A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase

OBJECTIVE: To change the specificity of a glutaryl-7-aminocephalosporanic acid acylase (GCA) towards N-acyl homoserine lactones (AHLs; quorum sensing signalling molecules) by site-directed mutagenesis. RESULTS: Seven residues were identified by analysis of existing crystal structures as potential de...

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Bibliografski detalji
Izdano u:Biotechnol Lett
Glavni autori: Murugayah, Shereen A., Evans, Gary B., Tyndall, Joel D. A., Gerth, Monica L.
Format: Artigo
Jezik:Inglês
Izdano: Springer Netherlands 2021
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8197700/
https://ncbi.nlm.nih.gov/pubmed/33891232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-021-03135-9
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