Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Martens, Yuka A., Xu, Siming, Tait, Richard, Li, Gary, Zhao, Xinping C., Lu, Wenyan, Liu, Chia-Chen, Kanekiyo, Takahisa, Bu, Guojun, Zhao, Jing
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8190508/
https://ncbi.nlm.nih.gov/pubmed/34151296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100571
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