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Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity
While bespoke sequence-specific proteases have the potential to advance biotechnology and medicine, generating proteases with tailor-made cleavage specificities remains a major challenge. We developed a phage-assisted protease evolution system with simultaneous positive and negative selection, and a...
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| Publicado no: | Science |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8175023/ https://ncbi.nlm.nih.gov/pubmed/33602850 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.abf5972 |
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