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Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains

Immunogold labeling allows localization of proteins at the electron microscopy (EM) level of resolution, and quantification of signals. The present paper summarizes methodological issues and experiences gained from studies on the distribution of synaptic and other neuron-specific proteins in cell cu...

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Detalhes bibliográficos
Publicado no:Mol Brain
Main Authors: Tao-Cheng, Jung-Hwa, Crocker, Virginia, Moreira, Sandra Lara, Azzam, Rita
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8173732/
https://ncbi.nlm.nih.gov/pubmed/34082785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13041-021-00799-2
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