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Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains
Immunogold labeling allows localization of proteins at the electron microscopy (EM) level of resolution, and quantification of signals. The present paper summarizes methodological issues and experiences gained from studies on the distribution of synaptic and other neuron-specific proteins in cell cu...
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| Udgivet i: | Mol Brain |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8173732/ https://ncbi.nlm.nih.gov/pubmed/34082785 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13041-021-00799-2 |
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