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Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes
Precise immunolocalization of proteins within a cell is central to understanding cell processes and functions such as intracellular trafficking and secretion of molecules during immune responses. Here we describe a protocol for ultrastructural detection of proteins in leukocytes. The method uses a p...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4204927/ https://ncbi.nlm.nih.gov/pubmed/25211515 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.163 |
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