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Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes

Precise immunolocalization of proteins within a cell is central to understanding cell processes and functions such as intracellular trafficking and secretion of molecules during immune responses. Here we describe a protocol for ultrastructural detection of proteins in leukocytes. The method uses a p...

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Bibliografiske detaljer
Main Authors: Melo, Rossana C N, Morgan, Ellen, Monahan-Earley, Rita, Dvorak, Ann M, Weller, Peter F
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4204927/
https://ncbi.nlm.nih.gov/pubmed/25211515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.163
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