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Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids

Mammary stem cells (MaSCs) contribute to mammary epithelium development and homeostasis. They have been proposed as cells of origin for breast cancer. Here, we describe an organoid culture protocol for ex vivo expansion of MaSCs from mouse tissues. These organoids maintain the self-renewal of gland-...

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Podrobná bibliografie
Vydáno v:STAR Protoc
Hlavní autoři: Cui, Jihong, Guo, Wenjun
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8173303/
https://ncbi.nlm.nih.gov/pubmed/34124696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100577
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