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Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids
Mammary stem cells (MaSCs) contribute to mammary epithelium development and homeostasis. They have been proposed as cells of origin for breast cancer. Here, we describe an organoid culture protocol for ex vivo expansion of MaSCs from mouse tissues. These organoids maintain the self-renewal of gland-...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8173303/ https://ncbi.nlm.nih.gov/pubmed/34124696 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100577 |
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