Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors

We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This long-term...

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Dettagli Bibliografici
Pubblicato in:STAR Protoc
Autori principali: Sinha, Sayantani, Chakraborty, Sayan, Sengupta, Amitava
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2020
Soggetti:
Protocol
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757360/
https://ncbi.nlm.nih.gov/pubmed/33377055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100161
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