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In silico Design of Linear DNA for Robust Cell-Free Gene Expression

Cell-free gene expression systems with linear DNA expression templates (LDETs) have been widely applied in artificial cells, biochips, and high-throughput screening. However, due to the degradation caused by native nucleases in cell extracts, the transcription with linear DNA templates is weak, ther...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Front Bioeng Biotechnol
Hauptverfasser: Chen, Xinjie, Lu, Yuan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8169995/
https://ncbi.nlm.nih.gov/pubmed/34095101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2021.670341
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